PIM2 Antibody

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ProSci
Product Code: PSI-63-295
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-295-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: SW620 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of anti-PIM2 Antibody in Hela cell line lysates (35ug/lane)
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PIM proteins were immunoprecipitated from MV4;11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of K00135. Phosphorylation of BAD (both on Ser112 and Ser136, detected by WB with phospho-specific antibodies) was abrogated on addition of the compound. Asterisks, strong bands corresponding to the heavy chain of the anti-PIM2 rabbit antibody recognized by the antirabbit immunoglobulin G secondary antibody. Beads alone (without anti-PIM antibodies) were incubated with the MV4;11 extract and used for the same in vitro phosphorylation reaction as a negative control.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: SW620 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-PIM2 Antibody in Hela cell line lysates (35ug/lane)
PIM proteins were immunoprecipitated from MV4;11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of K00135. Phosphorylation of BAD (both on Ser112 and Ser136, detected by WB with phospho-specific antibodies) was abrogated on addition of the compound. Asterisks, strong bands corresponding to the heavy chain of the anti-PIM2 rabbit antibody recognized by the antirabbit immunoglobulin G secondary antibody. Beads alone (without anti-PIM antibodies) were incubated with the MV4;11 extract and used for the same in vitro phosphorylation reaction as a negative control.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Serine/threonine-protein kinase pim-2, Pim-2h, PIM2
Application Note:
For WB starting dilution is: 1:1000

For IP starting dilution is: 1:50~100

For IHC-P starting dilution is: 1:50~100
Background:
Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases. Pim-2 is highly homologous to Pim-1 with similar oncogenic functions. Pim-2 overexpression promotes resistance to a host of apoptotic stimuli; its expression is negatively regulated by growth factor depletion. Increased levels of Pim-2 has also been observed in certain cancers.
Background References:
  • Yan, B., et al., J. Biol. Chem. 278(46):45358-45367 (2003).
  • Baytel, D., et al., Biochim. Biophys. Acta 1442 (2-3), 274-285 (1998).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-308 amino acids from the C-terminal region of human PIM2.
NCBI Gene ID #:
11040
NCBI Official Name:
Serine/threonine-protein kinase pim-2
NCBI Official Symbol:
PIM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
Q9P1W9
Protein GI Number:
20139243
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction
Swissprot #:
Q9P1W9
User NOte:
Optimal dilutions for each application to be determined by the researcher.