LGR5 Antibody

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ProSci
Product Code: PSI-61-474
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-474-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (loop1) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).
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Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (loop1) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).

Documents

Further Information

Additional Names:
Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5, GPR49, GPR67
Application Note:
For IHC-P starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues.
Background References:
  • Barker,N., Nature 449 (7165), 1003-1007 (2007) McClanahan,T., Cancer Biol. Ther.(4), 419-426 (2006) Yamamoto,Y., Hepatology 37 (3), 528-533 (2003) Hsu,S.Y., Mol. Endocrinol. 14 (8), 1257-1271 (2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This LGR5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 605-638 amino acids from human LGR5.
NCBI Gene ID #:
8549
NCBI Official Name:
Leucine-rich repeat-containing G-protein coupled receptor 5
NCBI Official Symbol:
LGR5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
100 kDa
Protein Accession #:
O75473
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction,Growth Factor
Swissprot #:
O75473
User NOte:
Optimal dilutions for each application to be determined by the researcher.