PMF1 Antibody

ProSci
Product Code: PSI-60-440
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-440-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.

Further Information

Additional Names:
Polyamine-modulated factor 1, PMF-1, PMF1 {ECO:0000312|EMBL:AAH650311}
Application Note:
For WB starting dilution is: 1:1000
Background:
Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.
Background References:
  • Aleman, A., et al. Clin. Cancer Res. 14(24):8236-8243(2008)
  • Kline, S.L., et al. J. Cell Biol. 173(1):9-17(2006)
  • Obuse, C., et al. Nat. Cell Biol. 6(11):1135-1141(2004)
  • Wang, Y., et al. Biochem. J. 366 (PT 1), 79-86 (2002) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PMF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-135 amino acids from the Central region of human PMF1.
NCBI Gene ID #:
100527963, 11243
NCBI Official Name:
Polyamine-modulated factor 1
NCBI Official Symbol:
PMF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
23 kDa
Protein Accession #:
Q6P1K2
Protein GI Number:
114152119
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q6P1K2
User NOte:
Optimal dilutions for each application to be determined by the researcher.