UBE2G1 Antibody

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ProSci
Product Code: PSI-58-992
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-992-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Y79 cell line lysates (35ug/lane).
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Western blot analysis of UBE2G1 using rabbit polyclonal UBE2G1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2G1 gene.
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Western blot analysis in Y79 cell line lysates (35ug/lane).
Western blot analysis of UBE2G1 using rabbit polyclonal UBE2G1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2G1 gene.

Documents

Further Information

Additional Names:
Ubiquitin-conjugating enzyme E2 G1, E217K, UBC7, Ubiquitin carrier protein G1, Ubiquitin-protein ligase G1, Ubiquitin-conjugating enzyme E2 G1, N-terminally processed, UBE2G1, UBE2G
Application Note:
For WB starting dilution is: 1:1000
Background:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein may be involved in degradation of muscle-specific proteins. [provided by RefSeq].
Background References:
  • Hassink, G., et al. Biochem. J. 388 (PT 2), 647-655 (2005) :
  • Kim, B.W., et al. Mol. Endocrinol. 17(12):2603-2612(2003)
  • Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003)
  • Imai, Y., et al. Cell 105(7):891-902(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey, Mouse, Rat
Immunogen:
This UBE2G1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-155 amino acids from the C-terminal region of human UBE2G1.
NCBI Gene ID #:
7326
NCBI Official Name:
Ubiquitin-conjugating enzyme E2 G1
NCBI Official Symbol:
UBE2G1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
P62253
Protein GI Number:
51338681
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
P62253
User NOte:
Optimal dilutions for each application to be determined by the researcher.