Cullin 4A Antibody
| Code | Size | Price |
|---|
| PSI-63-018-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Cullin-4A, CUL-4A, CUL4A
Application Note:
For WB starting dilution is: 1:1000
Background:
CUL4a is a core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitiantion of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9.
Background References:
- Huang,J.,Oncogene 27 (29), 4056-4064 (2008)
- Hu,J.,Genes Dev. 22 (7), 866-871 (2008)
- Wen,X., J. Biol. Chem. 282 (37), 27046-27057 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This Cullin 4A (CUL4A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-131 amino acids from the N-terminal region of human Cullin 4A (CUL4A).
NCBI Gene ID #:
8451
NCBI Official Name:
Cullin-4A
NCBI Official Symbol:
CUL4A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
88 kDa
Protein Accession #:
Q13619
Protein GI Number:
108936013
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
Q13619
User NOte:
Optimal dilutions for each application to be determined by the researcher.