POLA2 Antibody

ProSci
Product Code: PSI-59-099
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-59-099-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in T47D cell line lysates (35ug/lane).

Western blot analysis in T47D cell line lysates (35ug/lane).

Further Information

Additional Names:
DNA polymerase alpha subunit B, DNA polymerase alpha 70 kDa subunit, POLA2
Application Note:
For WB starting dilution is: 1:1000
Background:
POLA2 may play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity).
Background References:
  • Michiels, S., et al. Carcinogenesis 30(5):763-768(2009)
  • Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
  • Stelzl, U., et al. Cell 122(6):957-968(2005)
  • Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This POLA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-240 amino acids from the Central region of human POLA2.
NCBI Gene ID #:
23649
NCBI Official Name:
DNA polymerase alpha subunit B
NCBI Official Symbol:
POLA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
Q14181
Protein GI Number:
90110415
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q14181
User NOte:
Optimal dilutions for each application to be determined by the researcher.