Mouse Wee1 Antibody
| Code | Size | Price |
|---|
| PSI-60-265-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Wee1-like protein kinase, Wee1A kinase, Wee1
Application Note:
For WB starting dilution is: 1:1000
Background:
Wee1 may act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur (By similarity).
Background References:
- Muller, M., et al. J. Cell. Sci. 123 (PT 2), 286-294 (2010) :
- Kim, M.J., et al. Oncol. Rep. 19(5):1323-1329(2008)
- Tanaka, Y., et al. Biochem. Biophys. Res. Commun. 352(1):21-28(2007)
- Tominaga, Y., et al. Int. J. Biol. Sci. 2(4):161-170(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This Mouse Wee1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-230 amino acids from the Central region of mouse Wee1.
NCBI Gene ID #:
22390
NCBI Official Name:
Wee1-like protein kinase
NCBI Official Symbol:
Wee1
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
72 kDa
Protein Accession #:
P47810
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
P47810
User NOte:
Optimal dilutions for each application to be determined by the researcher.