UCHL5 Antibody

ProSci
Product Code: PSI-60-847
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-847-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

Further Information

Additional Names:
Ubiquitin carboxyl-terminal hydrolase isozyme L5, UCH-L5, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin thioesterase L5, UCHL5, UCH37
Application Note:
For WB starting dilution is: 1:1000
Background:
Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome.
Background References:
  • Nishio, K., et al. Biochem. Biophys. Res. Commun. 390(3):855-860(2009)
  • Yao, T., et al. Mol. Cell 31(6):909-917(2008)
  • Koulich, E., et al. Mol. Biol. Cell 19(3):1072-1082(2008)
  • Qiu, X.B., et al. EMBO J. 25(24):5742-5753(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This UCHL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from the Central region of human UCHL5.
NCBI Gene ID #:
51377
NCBI Official Name:
Ubiquitin carboxyl-terminal hydrolase isozyme L5
NCBI Official Symbol:
UCHL5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
Q9Y5K5
Protein GI Number:
108936023
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9Y5K5
User NOte:
Optimal dilutions for each application to be determined by the researcher.