USP21 Antibody

ProSci
Product Code: PSI-60-978
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-978-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of hUSP21-L241 in NCI-H460 cell line lysates (35ug/lane)

Western blot analysis of hUSP21-L241 in NCI-H460 cell line lysates (35ug/lane)

Further Information

Additional Names:
Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23
Application Note:
For WB starting dilution is: 1:1000
Background:
USP21 is a ubiquitin-specific protease, an enzyme that removes ubiquitin from ubiquitinated proteins. The encoded protein belongs to the C19 peptidase family, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. This protein has been reported to be capable of removing NEDD8 from NEDD8 conjugates.
Background References:
  • Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003).
  • Gong, L., et al., J. Biol. Chem. 275(19):14212-14216 (2000).
  • Hillier, L.D., et al., Genome Res. 6(9):807-828 (1996).
  • Smith, T.S., et al., Biochim. Biophys. Acta 1490 (1-2), 184-188 (2000).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This USP21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-256 amino acids from the Central region of human USP21.
NCBI Gene ID #:
27005
NCBI Official Name:
Ubiquitin carboxyl-terminal hydrolase 21
NCBI Official Symbol:
USP21
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
63 kDa
Protein Accession #:
Q9UK80
Protein GI Number:
10720334
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
Q9UK80
User NOte:
Optimal dilutions for each application to be determined by the researcher.