NCAPD3 Antibody

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ProSci
Product Code: PSI-58-720
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-720-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

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Western blot analysis in K562 cell line lysates (35ug/lane).
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Western blot analysis in K562 cell line lysates (35ug/lane).

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Further Information

Additional Names:
Condensin-2 complex subunit D3, Non-SMC condensin II complex subunit D3, hCAP-D3, NCAPD3, CAPD3, KIAA0056
Application Note:
For WB starting dilution is: 1:1000
Background:
Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 (MIM 605576) and SMC4 (MIM 605575), but they contain different sets of non-SMC subunits. NCAPD3 is 1 of 3 non-SMC subunits that define condensin II (Ono et al., 2003 [PubMed 14532007]).
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Takemoto, A., et al. Nat. Struct. Mol. Biol. 16(12):1302-1308(2009)
  • Lapointe, J., et al. Am. J. Surg. Pathol. 32(2):205-209(2008)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NCAPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1050-1078 amino acids from the C-terminal region of human NCAPD3.
NCBI Gene ID #:
23310
NCBI Official Name:
Condensin-2 complex subunit D3
NCBI Official Symbol:
NCAPD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
169 kDa
Protein Accession #:
P42695
Protein GI Number:
82654946
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
P42695
User NOte:
Optimal dilutions for each application to be determined by the researcher.