NASP Antibody

ProSci
Product Code: PSI-61-706
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-706-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: human testis lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of NASP using rabbit polyclonal NASP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NASP gene (Lane 2).
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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: human testis lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of NASP using rabbit polyclonal NASP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NASP gene (Lane 2).

Further Information

Additional Names:
Nuclear autoantigenic sperm protein, NASP, NASP
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
NASP encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
Background References:
  • Alekseev, O.M., et al. Reprod. Biol. Endocrinol. 7, 45 (2009)
  • Wang, H., et al. Nucleic Acids Res. 36(18):5763-5772(2008)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NASP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-180 amino acids from the N-terminal region of human NASP.
NCBI Gene ID #:
4678
NCBI Official Name:
Nuclear autoantigenic sperm protein
NCBI Official Symbol:
NASP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
85 kDa
Protein Accession #:
P49321
Protein GI Number:
23503077
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
P49321
User NOte:
Optimal dilutions for each application to be determined by the researcher.