ARSF Antibody

ProSci
Product Code: PSI-56-948
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-948-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis in K562 cell line lysates (35ug/lane).
2 / 3
Western blot analysis of ARSF using rabbit polyclonal ARSF Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARSF gene.
3 / 3

Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis of ARSF using rabbit polyclonal ARSF Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARSF gene.

Further Information

Additional Names:
Arylsulfatase F, ASF, 316-, ARSF
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This gene is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin.
Background References:
  • Cao, W., et al. J. Biol. Chem. 273(32):20629-20635(1998)
  • Puca, A.A., et al. Genomics 42(2):192-199(1997)
  • Parenti, G., et al. Curr. Opin. Genet. Dev. 7(3):386-391(1997)
  • Franco, B., et al. Cell 81(1):15-25(1995)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ARSF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the Central region of human ARSF.
NCBI Gene ID #:
416
NCBI Official Name:
Arylsulfatase F
NCBI Official Symbol:
ARSF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
P54793
Protein GI Number:
259016386
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
P54793
User NOte:
Optimal dilutions for each application to be determined by the researcher.