CF150 Antibody

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ProSci
Product Code: PSI-55-229
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-229-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing U-2OS cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Flow cytometric analysis of A549 cells using CF150 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Western blot analysis of lysates from MCF-7, HT-29, THP-1 cell line, human spleen, human cerebellum tissue lysate(from left to right), using CF150 Antibody at 1:1000 at each lane.
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Western blot analysis of lysates from HT-29, THP-1 cell line, human cerebellum tissue lysate (from left to right), using CF150 Antibody at 1:1000 at each lane.
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Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Overlay histogram showing U-2OS cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Flow cytometric analysis of A549 cells using CF150 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from MCF-7, HT-29, THP-1 cell line, human spleen, human cerebellum tissue lysate(from left to right), using CF150 Antibody at 1:1000 at each lane.
Western blot analysis of lysates from HT-29, THP-1 cell line, human cerebellum tissue lysate (from left to right), using CF150 Antibody at 1:1000 at each lane.
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Cyclic GMP-AMP synthase, cGAMP synthase, cGAS, h-cGAS, Mab-21 domain-containing protein 1, MB21D1, C6orf150
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:500-1:1000
Background:
The exact function of C6orf150 remains unknown.
Background References:
  • Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
  • Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
  • Mungall, A.J., et al. Nature 425(6960):805-811(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CF150 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human CF150.
NCBI Gene ID #:
115004
NCBI Official Name:
Cyclic GMP-AMP synthase
NCBI Official Symbol:
MB21D1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
59 kDa
Protein Accession #:
Q8N884
Protein GI Number:
68565218
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q8N884
User NOte:
Optimal dilutions for each application to be determined by the researcher.