NAT13 Antibody

ProSci
Product Code: PSI-61-775
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-775-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
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Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
N-alpha-acetyltransferase 50, 231-, N-acetyltransferase 13, N-acetyltransferase 5, hNAT5, N-acetyltransferase san homolog, hSAN, NatE catalytic subunit, NAA50, MAK3, NAT13, NAT5
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
NAT13 is probable catalytic component of the ARD1A-NARG1 complex which displays alpha (N-terminal) acetyltransferase activity.
Background References:
  • Starheim, K.K., et al. Mol. Cell. Biol. 29(13):3569-3581(2009)
  • Polevoda, B., et al. BMC ProcSUPPL 6, S2 (2009)
  • Hou, F., et al. J. Cell Biol. 177(4):587-597(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Zebrafish, Mouse, Xenopus
Immunogen:
This NAT13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 amino acids from the C-terminal region of human NAT13.
NCBI Gene ID #:
80218
NCBI Official Name:
N-alpha-acetyltransferase 50
NCBI Official Symbol:
NAA50
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
Q9GZZ1
Protein GI Number:
74733509
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9GZZ1
User NOte:
Optimal dilutions for each application to be determined by the researcher.