RAD17 Antibody
| Code | Size | Price |
|---|
| PSI-55-996-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Flow Cytometry
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog, RAD17, R24L
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Background:
The protein encoded by this gene is highly similar to the
gene product of Schizosaccharomyces pombe rad17, a cell cycle
checkpoint gene required for cell cycle arrest and DNA damage
repair in response to DNA damage. This protein shares strong
similarity with DNA replication factor C (RFC), and can form a
complex with RFCs. This protein binds to chromatin prior to DNA
damage and is phosphorylated by the checkpoint kinase ATR following
damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein
complex onto chromatin after DNA damage, which may be required for
its phosphorylation. The phosphorylation of this protein is
required for the DNA-damage-induced cell cycle G2 arrest, and is
thought to be a critical early event during checkpoint signaling in
DNA-damaged cells. Eight alternatively spliced transcript variants
of this gene, which encode four distinct proteins, have been
reported. Two pseudogenes, located on chromosomes 7 and 13, have
been identified.
Background References:
- Zhang, L., et al. EMBO J. 29(10):1726-1737(2010)
- Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)
- Beretta, G.L., et al. Cancer Lett. 266(2):194-202(2008)
- Zhao, M., et al. Head Neck 30(1):35-42(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RAD17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human RAD17.
NCBI Gene ID #:
5884
NCBI Official Name:
Cell cycle checkpoint protein RAD17
NCBI Official Symbol:
RAD17
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
77 kDa
Protein Accession #:
O75943
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
O75943
User NOte:
Optimal dilutions for each application to be determined by the researcher.