FAM50A Antibody

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ProSci
Product Code: PSI-60-987
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-987-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Fluorescent image of Hela cells stained with FAM50A Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
2 / 2
Western blot analysis in A549,Hela cell line lysates (35ug/lane).
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Fluorescent image of Hela cells stained with FAM50A Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis in A549,Hela cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Protein FAM50A, Protein HXC-26, Protein XAP-5, FAM50A, DXS9928E, HXC26, XAP5
Application Note:
For IF starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
May be a DNA-binding protein or transcriptional factor.
Background References:
  • Mazzarella R., et al. Genomics 45:216-219(1997).
  • Ota T., et al. Nat. Genet. 36:40-45(2004).
  • Ross M.T., et al. Nature 434:325-337(2005).
  • Mural R.J., et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Zebrafish, Mouse
Immunogen:
This FAM50A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 311-339 amino acids from the C-terminal region of human FAM50A.
NCBI Gene ID #:
9130
NCBI Official Name:
Protein FAM50A
NCBI Official Symbol:
FAM50A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
Q14320
Protein GI Number:
12231058
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q14320
User NOte:
Optimal dilutions for each application to be determined by the researcher.