ZHX2 Antibody

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ProSci
Product Code: PSI-60-104
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-104-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
2 / 2
Fluorescent confocal image of A549 cell stained with ZHX2 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZHX2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZHX2 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.
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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
Fluorescent confocal image of A549 cell stained with ZHX2 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZHX2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZHX2 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.

Documents

Further Information

Additional Names:
Zinc fingers and homeoboxes protein 2, Alpha-fetoprotein regulator 1, AFP regulator 1, Regulator of AFP, Zinc finger and homeodomain protein 2, ZHX2, AFR1, KIAA0854, RAF
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Xu, J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(5):2136-2140(2010)
  • Trynka, G., et al. Gut 58(8):1078-1083(2009)
  • Shen, H., et al. J. Cell. Mol. Med. 12 (6B), 2772-2780 (2008) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ZHX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-131 amino acids from the N-terminal region of human ZHX2.
NCBI Gene ID #:
22882
NCBI Official Name:
Zinc fingers and homeoboxes protein 2
NCBI Official Symbol:
ZHX2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
92 kDa
Protein Accession #:
Q9Y6X8
Protein GI Number:
44888553
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9Y6X8
User NOte:
Optimal dilutions for each application to be determined by the researcher.