MyoD1 Antibody

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ProSci
Product Code: PSI-56-563
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-563-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: HL-60 whole cell lysate Lane 2: RD whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in Hela cell line and human placenta tissue lysates (35ug/lane).
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Western Blot at 1:1000 dilution Lane 1: HL-60 whole cell lysate Lane 2: RD whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Hela cell line and human placenta tissue lysates (35ug/lane).

Documents

Further Information

Additional Names:
Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, bHLHc1, Myogenic factor 3, Myf-3, MYOD1, BHLHC1, MYF3, MYOD
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
Background References:
  • Xynos, A., et al. Stem Cells 28(5):965-973(2010)
  • Stuelsatz, P., et al. J. Biol. Chem. 285(17):12670-12683(2010)
  • Hiraoka, S., et al. Hum. Pathol. 41(1):38-47(2010)
  • Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MyoD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human MyoD1.
NCBI Gene ID #:
4654
NCBI Official Name:
Myoblast determination protein 1
NCBI Official Symbol:
MYOD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
P15172
Protein GI Number:
209572729
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
P15172
User NOte:
Optimal dilutions for each application to be determined by the researcher.