HN1 Antibody

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ProSci
Product Code: PSI-57-996
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-996-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in 293 cell line lysates (35ug/lane).
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Confocal immunofluorescent analysis of HN1 Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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Western blot analysis in 293 cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of HN1 Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Hematological and neurological expressed 1 protein, Androgen-regulated protein 2, Hematological and neurological expressed 1 protein, N-terminally processed, HN1, ARM2
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
HN1 belongs to the HN1 family.
Background References:
  • Laughlin, K.M., et al. Pathol. Oncol. Res. 15(3):437-444(2009)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Zougman, A., et al. J. Proteome Res. 5(4):925-934(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the Central region of human HN1.
NCBI Gene ID #:
51155
NCBI Official Name:
Hematological and neurological expressed 1 protein
NCBI Official Symbol:
HN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
16 kDa
Protein Accession #:
Q9UK76
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9UK76
User NOte:
Optimal dilutions for each application to be determined by the researcher.