CCDC99 Antibody
| Code | Size | Price |
|---|
| PSI-57-083-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Protein Spindly {ECO:0000255|HAMAP-Rule:MF_03041}, hSpindly, Arsenite-related gene 1 protein, Coiled-coil domain-containing protein 99 {ECO:0000255|HAMAP-Rule:MF_03041}, Rhabdomyosarcoma antigen MU-RMS-404A, Spindle apparatus coiled-coil domain-containing protein 1 {ECO:0000255|HAMAP-Rule:MF_03041}, SPDL1 {ECO:0000255|HAMAP-Rule:MF_03041}
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
CCDC99 is required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
Background References:
- Barisic, M., et al. Mol. Biol. Cell 21(12):1968-1981(2010)
- Gassmann, R., et al. Genes Dev. 24(9):957-971(2010)
- Chan, Y.W., et al. J. Cell Biol. 185(5):859-874(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This CCDC99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 459-488 amino acids from the C-terminal region of human CCDC99.
NCBI Gene ID #:
54908
NCBI Official Name:
Protein Spindly {ECO:0000255|HAMAP-Rule:MF_03041}
NCBI Official Symbol:
SPDL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
70 kDa
Protein Accession #:
Q96EA4
Protein GI Number:
296452913
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q96EA4
User NOte:
Optimal dilutions for each application to be determined by the researcher.