MDM1 Antibody

ProSci
Product Code: PSI-56-361
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-361-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A549 cell line lysates (35ug/lane).
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MDM1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in A549 cell line lysates (35ug/lane).
MDM1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Nuclear protein MDM1, MDM1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles and is amplified in the mouse transformed 3T3 cell line, and the protein is able to bind to p53. In mouse several transcripts have been described for this gene which result from alternative polyadenylation, splicing and exon usage.
Background References:
  • Chang, B., et al. Hum. Mol. Genet. 17(24):3929-3941(2008)
  • Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
  • Snyder, L.C., et al. J. Biol. Chem. 263(32):17150-17158(1988)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MDM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656-684 amino acids from the C-terminal region of human MDM1.
NCBI Gene ID #:
56890
NCBI Official Name:
Nuclear protein MDM1
NCBI Official Symbol:
MDM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
81 kDa
Protein Accession #:
Q8TC05
Protein GI Number:
156632525
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q8TC05
User NOte:
Optimal dilutions for each application to be determined by the researcher.