OTUD1 Antibody

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ProSci
Product Code: PSI-55-683
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-683-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
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OTUD1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
OTUD1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
OTU domain-containing protein 1, DUBA-7, OTUD1, DUBA7, OTDC1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Deubiquitinating enzymes (DUBs; see MIM 603478) are proteases that specifically cleave ubiquitin (MIM 191339) linkages, negating the action of ubiquitin ligases. DUBA7 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.[supplied by OMIM].
Background References:
  • Franke, A., et al. Gastroenterology 135(4):1207-1215(2008)
  • Kayagaki, N., et al. Science 318(5856):1628-1632(2007)
  • Deloukas, P., et al. Nature 429(6990):375-381(2004)
  • Makarova, K.S., et al. Trends Biochem. Sci. 25(2):50-52(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This OTUD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282-311 amino acids from the Central region of human OTUD1.
NCBI Gene ID #:
220213
NCBI Official Name:
OTU domain-containing protein 1
NCBI Official Symbol:
OTUD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
Q5VV17
Protein GI Number:
74747188
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q5VV17
User NOte:
Optimal dilutions for each application to be determined by the researcher.