SENP1 Antibody

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ProSci
Product Code: PSI-56-338
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-338-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of SENP1 polyclonal antibody in whole HL60 cell lysate: SENP1 was detected using purified Pab.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of SENP1 polyclonal antibody in whole HL60 cell lysate: SENP1 was detected using purified Pab.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Sentrin-specific protease 1, Sentrin/SUMO-specific protease SENP1, SENP1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
SENP1 is a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. SENP deconjugates SUMO1 from HIPK2 and from HDAC1, which decreases the transcriptional repression activity of the latter.
Background References:
  • Gong, L., et al., J. Biol. Chem. 275(5):3355-3359 (2000). Bailey, D., et al., J. Gen. Virol. 83 (Pt 12), 2951-2964 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SENP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-59 amino acids from the N-terminal region of human SENP1.
NCBI Gene ID #:
29843
NCBI Official Name:
Sentrin-specific protease 1
NCBI Official Symbol:
SENP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
73 kDa
Protein Accession #:
Q9P0U3
Protein GI Number:
215273882
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
Q9P0U3
User NOte:
Optimal dilutions for each application to be determined by the researcher.