MAGED4B Antibody

ProSci
Product Code: PSI-57-050
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-050-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HepG2 cell line lysates (35ug/lane).
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MAGED4B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in HepG2 cell line lysates (35ug/lane).
MAGED4B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Melanoma-associated antigen D4, MAGE-D4 antigen, MAGE-E1 antigen, MAGED4, KIAA1859, MAGED4A, MAGEE1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene is a member of the MAGED gene family. It is expressed only in brain and ovary among normal tissues, and two transcript variants of this gene are specifically expressed in glioma cells among cancer cells. This gene and the other MAGED genes are clustered on chromosome Xp11. Multiple alternatively spliced transcript variants have been found for this gene, however, the full-length nature of some variants has not been defined.
Background References:
  • Ito, S., et al. Lung Cancer 51(1):79-88(2006)
  • Kawano, Y., et al. Gene 277 (1-2), 129-137 (2001) :
  • Sasaki, M., et al. Cancer Res. 61(12):4809-4814(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This MAGED4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 328-357 amino acids from the Central region of human MAGED4B.
NCBI Gene ID #:
728239, 81557
NCBI Official Name:
Melanoma-associated antigen D4
NCBI Official Symbol:
MAGED4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
81 kDa
Protein Accession #:
Q96JG8
Protein GI Number:
41017391
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q96JG8
User NOte:
Optimal dilutions for each application to be determined by the researcher.