LCE1A Antibody

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ProSci
Product Code: PSI-55-492
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-492-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in T47D cell line lysates (35ug/lane).
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LCE1A antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in T47D cell line lysates (35ug/lane).
LCE1A antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Late cornified envelope protein 1A, Late envelope protein 1, LCE1A, LEP1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
LCE1A belongs to the late cornified envelope (LCE) gene cluster within the epidermal differentiation complex (EDC) on chromosome 1. The LCE cluster contains multiple conserved genes that encode stratum corneum proteins, and these genes are expressed relatively late during fetal assembly of the skin cornified envelope (Jackson et al., 2005 [PubMed 15854049]). For further information on the LCE gene cluster, see GENE FAMILY below.
Background References:
  • Jackson, B., et al. J. Invest. Dermatol. 124(5):1062-1070(2005)
  • Marshall, D., et al. Proc. Natl. Acad. Sci. U.S.A. 98(23):13031-13036(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This LCE1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the C-terminal region of human LCE1A.
NCBI Gene ID #:
353131
NCBI Official Name:
Late cornified envelope protein 1A
NCBI Official Symbol:
LCE1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
11 kDa
Protein Accession #:
Q5T7P2
Protein GI Number:
74745413
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q5T7P2
User NOte:
Optimal dilutions for each application to be determined by the researcher.