TRIM4 Antibody

ProSci
Product Code: PSI-56-971
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-971-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A549,293,HepG2 cell line lysates (35ug/lane).
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TRIM4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in A549,293,HepG2 cell line lysates (35ug/lane).
TRIM4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
E3 ubiquitin-protein ligase TRIM4, 632-, RING finger protein 87, Tripartite motif-containing protein 4, TRIM4, RNF87
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternatively spliced transcript variants that encode different isoforms have been described.
Background References:
  • Kasyapa, C., et al. Proteomics 9(16):3979-3988(2009)
  • Li, X., et al. Virology 360(2):419-433(2007)
  • Thompson, E.E., et al. Pharmacogenomics J. 6(2):105-114(2006)
  • Reymond, A., et al. EMBO J. 20(9):2140-2151(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TRIM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-141 amino acids from the N-terminal region of human TRIM4.
NCBI Gene ID #:
89122
NCBI Official Name:
E3 ubiquitin-protein ligase TRIM4
NCBI Official Symbol:
TRIM4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
Q9C037
Protein GI Number:
209572691
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9C037
User NOte:
Optimal dilutions for each application to be determined by the researcher.