ProSci

WEE1 Antibody

Product Code:
 
PSI-63-400
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 3
Western blot analysis in A431,Jurkat cell line lysates (35ug/lane).This demonstrates the WEE1 antibody detected the WEE1 protein (arrow).
3 / 3
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Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in A431,Jurkat cell line lysates (35ug/lane).This demonstrates the WEE1 antibody detected the WEE1 protein (arrow).

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PSI-63-400-400ul400ul£626.00
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Further Information

Additional Names:
Wee1-like protein kinase, WEE1hu, Wee1A kinase, WEE1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
WEE1 is a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
Background References:
  • Kawasaki, H., et al., Oncogene 22(44):6839-6844 (2003).
  • Hashimoto, O., et al., Mol. Carcinog. 36(4):171-182 (2003).
  • Yuan, H., et al., J. Virol. 77(3):2063-2070 (2003).
  • Masaki, T., et al., Hepatology 37(3):534-543 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This WEE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the Central region of human WEE1.
NCBI Gene ID #:
7465
NCBI Official Name:
Wee1-like protein kinase
NCBI Official Symbol:
WEE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
72 kDa
Protein Accession #:
P30291
Protein GI Number:
1351419
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
P30291
User NOte:
Optimal dilutions for each application to be determined by the researcher.