ProSci

CARD6 Antibody

Product Code:
 
PSI-63-757
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 7
Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
2 / 7
Immunohistochemical analysis of paraffin-embedded H. colon section using CARD6 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
3 / 7
4 / 7
Western blot analysis of lysate from WiDr cell line, using CARD6 Antibody at 1:1000.
5 / 7
Western blot analysis of lysates from A549, HepG2 cell line (from left to right), using CARD6 Antibody at 1:1000 at each lane.
6 / 7
Western blot analysis in WiDr cell line lysates (35ug/lane).
7 / 7
Western blot analysis of CARD6 Antibody in MDA-MB231 cell line lysates (35ug/lane)
❮❯

Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. colon section using CARD6 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis of lysate from WiDr cell line, using CARD6 Antibody at 1:1000.
Western blot analysis of lysates from A549, HepG2 cell line (from left to right), using CARD6 Antibody at 1:1000 at each lane.
Western blot analysis in WiDr cell line lysates (35ug/lane).
Western blot analysis of CARD6 Antibody in MDA-MB231 cell line lysates (35ug/lane)

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-63-757-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
Caspase recruitment domain-containing protein 6, CARD6
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
CARD6 is a protein that contains a caspase recruitment domain (CARD), an antiparallel six-helical bundle that mediates homotypic protein-protein interactions. The encoded protein is a microtubule-associated protein that has been shown to interact with receptor-interacting protein kinases and positively modulate signal transduction pathways converging on activation of the inducible transcription factor NF-kB.
Background References:
  • Kim,S.S., et.al., Pathology 42 (1), 50-53 (2010)
  • Olsen,J.V., et.al., Cell 127 (3), 635-648 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CARD6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human CARD6.
NCBI Gene ID #:
84674
NCBI Official Name:
Caspase recruitment domain-containing protein 6
NCBI Official Symbol:
CARD6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
116 kDa
Protein Accession #:
Q9BX69
Protein GI Number:
209572595
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9BX69
User NOte:
Optimal dilutions for each application to be determined by the researcher.