ProSci

TRMT2B Antibody

Product Code:
 
PSI-55-681
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in K562 cell line lysates (35ug/lane).
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TRMT2B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in K562 cell line lysates (35ug/lane).
TRMT2B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-55-681-400ul400ul£626.00
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Further Information

Additional Names:
tRNA (uracil(54)-C(5))-methyltransferase homolog, TRM2 homolog, TRMT2B, CXorf34
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
This gene encodes a homolog of the TRM2 gene in S. cerevisiae. The yeast gene encodes a tRNA methyltransferase that plays a role in tRNA maturation. The yeast protein also has endo-exonuclease activity and may be involved in DNA double strand break repair. Alternative splicing results in multiple transcripts encoding different isoforms.
Background References:
  • Kimura, K., et al. Genome Res. 16(1):55-65(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TRMT2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-165 amino acids from the N-terminal region of human TRMT2B.
NCBI Gene ID #:
79979
NCBI Official Name:
tRNA (uracil(54)-C(5))-methyltransferase homolog
NCBI Official Symbol:
TRMT2B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
56 kDa
Protein Accession #:
Q96GJ1
Protein GI Number:
74762656
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q96GJ1
User NOte:
Optimal dilutions for each application to be determined by the researcher.