ZP4 Antibody
| Code | Size | Price |
|---|
| PSI-56-605-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Zona pellucida sperm-binding protein 4, Zona pellucida glycoprotein 4, Zp-4, Zona pellucida protein B, Processed zona pellucida sperm-binding protein 4, ZP4, ZPB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
The zona pellucida is an extracellular matrix that
surrounds the oocyte and early embryo. It is composed primarily of
three or four glycoproteins with various functions during
fertilization and preimplantation development. The nascent protein
contains a N-terminal signal peptide sequence, a conserved ZP
domain, a consensus furin cleavage site, and a C-terminal
transmembrane domain. It is hypothesized that furin cleavage
results in release of the mature protein from the plasma membrane
for subsequent incorporation into the zona pellucida matrix.
However, the requirement for furin cleavage in this process remains
controversial based on mouse studies. Previously, this gene has
been referred to as ZP1 or ZPB and thought to have similar
functions as mouse Zp1. However, a human gene with higher
similarity and chromosomal synteny to mouse Zp1 has been assigned
the symbol ZP1 and this gene has been assigned the symbol ZP4.
Background References:
- Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
- McCauley, J.L., et al. Genes Immun. 10(7):624-630(2009)
- Nakano, M., et al. Proc. Natl. Acad. Sci. U.S.A. 106(31):12838-12842(2009)
- Choudhury, S., et al. J. Reprod. Immunol. 79(2):137-147(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ZP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from the C-terminal region of human ZP4.
NCBI Gene ID #:
57829
NCBI Official Name:
Zona pellucida sperm-binding protein 4
NCBI Official Symbol:
ZP4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
59 kDa
Protein Accession #:
Q12836
Protein GI Number:
46397080
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q12836
User NOte:
Optimal dilutions for each application to be determined by the researcher.