FAU Antibody

ProSci
Product Code: PSI-58-198
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-198-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-FUBI Center Pab (AP1600c) in Hela and T47D cell line lysates.
2 / 2
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with FUBI Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot analysis of anti-FUBI Center Pab (AP1600c) in Hela and T47D cell line lysates.
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with FUBI Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Ubiquitin-like protein FUBI, FAU
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
This gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome. Pseudogenes derived from this gene are present in the genome. Similar to ribosomal protein S30, ribosomal proteins S27a and L40 are synthesized as fusion proteins with ubiquitin.
Background References:
  • Rossman, T.G., et al., Oncogene 22(12):1817-1821 (2003).
  • Kenmochi, N., et al., Genome Res. 8(5):509-523 (1998).
  • Vladimirov, S.N., et al., Eur. J. Biochem. 239(1):144-149 (1996).
  • Kas, K., et al., Genomics 17(2):387-392 (1993).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Hamster, Mouse, Pig, Rat
Immunogen:
This FAU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-93 amino acids from the center region of human FUBI.
NCBI Gene ID #:
2197
NCBI Official Name:
Ubiquitin-like protein FUBI
NCBI Official Symbol:
FAU
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
8 kDa
Protein Accession #:
P35544
Protein GI Number:
549147
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
P35544
User NOte:
Optimal dilutions for each application to be determined by the researcher.