SEPSH2 Antibody

ProSci
Product Code: PSI-62-714
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-714-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-SEPSH2 Pabin HepG2 cell line lysate (35ug/lane).
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-SEPSH2 Pabin HepG2 cell line lysate (35ug/lane).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Further Information

Additional Names:
Selenide, water dikinase 2, Selenium donor protein 2, Selenophosphate synthase 2, SEPHS2, SPS2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
This protein encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem-loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop.
Background References:
  • Tamura, T., et al., Proc. Natl. Acad. Sci. U.S.A. 101(46):16162-16167 (2004).
  • Lescure, A., et al., J. Biol. Chem. 274(53):38147-38154 (1999).
  • Guimaraes, M.J., et al., Proc. Natl. Acad. Sci. U.S.A. 93(26):15086-15091 (1996).
  • Guimaraes, M.J., et al., Development 121(10):3335-3346 (1995).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SEPSH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-201 amino acids from the Central region of human SEPSH2.
NCBI Gene ID #:
22928
NCBI Official Name:
Selenide, water dikinase 2
NCBI Official Symbol:
SEPHS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
Q99611
Protein GI Number:
172044671
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
Q99611
User NOte:
Optimal dilutions for each application to be determined by the researcher.