NUSAP Antibody

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ProSci
Product Code: PSI-55-549
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-549-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in U251 cell line lysates (35ug/lane).
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NUSAP antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in U251 cell line lysates (35ug/lane).
NUSAP antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Nucleolar and spindle-associated protein 1, NuSAP, NUSAP1, ANKT
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
NUSAP1 is a nucleolar-spindle-associated protein that plays a role in spindle microtubule organization (Raemaekers et al., 2003 [PubMed 12963707]).
Background References:
  • Wadia, P.P., et al. Blood 115(10):2077-2087(2010)
  • Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
  • Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
  • Raemaekers, T., et al. J. Cell Biol. 162(6):1017-1029(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NUSAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the N-terminal region of human NUSAP.
NCBI Gene ID #:
51203
NCBI Official Name:
Nucleolar and spindle-associated protein 1
NCBI Official Symbol:
NUSAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
Q9BXS6
Protein GI Number:
74717631
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9BXS6
User NOte:
Optimal dilutions for each application to be determined by the researcher.