PAPSS2 Antibody

ProSci
Product Code: PSI-63-391
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-391-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Antibody is used in Western blot to detect PAPSS2 in Jurkat cell lysate.
2 / 2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Antibody is used in Western blot to detect PAPSS2 in Jurkat cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Further Information

Additional Names:
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2, PAPS synthase 2, PAPSS 2, Sulfurylase kinase 2, SK 2, SK2, Sulfate adenylyltransferase, ATP-sulfurylase, Sulfate adenylate transferase, SAT, Adenylyl-sulfate kinase, 3'-phosphoadenosine-5'-phosphosulfate synthase, APS kinase, Adenosine-5'-phosphosulfate 3'-phosphotransferase, Adenylylsulfate 3'-phosphotransferase, PAPSS2, ATPSK2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes. SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2.
Background References:
  • Xu, Z.H., et al., Biochem. Biophys. Res. Commun. 268(2):437-444 (2000).
  • Kurima, K., et al., J. Biol. Chem. 274(47):33306-33312 (1999).
  • ul Haque, M.F., et al., Nat. Genet. 20(2):157-162 (1998).
  • Kurima, K., et al., Proc. Natl. Acad. Sci. U.S.A. 95(15):8681-8685 (1998).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PAPSS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 544-574 amino acids from the C-terminal region of human PAPSS2.
NCBI Gene ID #:
9060
NCBI Official Name:
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2
NCBI Official Symbol:
PAPSS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
70 kDa
Protein Accession #:
O95340
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
O95340
User NOte:
Optimal dilutions for each application to be determined by the researcher.