RANGAP1 Antibody

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ProSci
Product Code: PSI-57-264
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-264-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Hela cell line lysates (35ug/lane).
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Western blot analysis of RANGAP1 using rabbit polyclonal RANGAP1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RANGAP1 gene.
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Western blot analysis in Hela cell line lysates (35ug/lane).
Western blot analysis of RANGAP1 using rabbit polyclonal RANGAP1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RANGAP1 gene.

Documents

Further Information

Additional Names:
Ran GTPase-activating protein 1, RanGAP1, RANGAP1, KIAA1835, SD
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.
Background References:
  • Zhang, J., et al. Biochem. Biophys. Res. Commun. 375(2):252-255(2008)
  • Zuccolo, M., et al. EMBO J. 26(7):1853-1864(2007)
  • Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
  • Vertegaal, A.C., et al. Mol. Cell Proteomics 5(12):2298-2310(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RANGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-49 amino acids from the N-terminal region of human RANGAP1.
NCBI Gene ID #:
5905
NCBI Official Name:
Ran GTPase-activating protein 1
NCBI Official Symbol:
RANGAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
64 kDa
Protein Accession #:
P46060
Protein GI Number:
1172922
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
P46060
User NOte:
Optimal dilutions for each application to be determined by the researcher.