CUL5 Antibody
| Code | Size | Price |
|---|
| PSI-63-007-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Cullin-5, CUL-5, Vasopressin-activated calcium-mobilizing receptor 1, VACM-1, CUL5, VACM1
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
Background:
CUL5 is a core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAk2. It seems to be involved poteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein and may form a cell surface vasopressin receptor.
Background References:
- Kamura T., Burian D., Yan Q.J. Biol. Chem. 276:29748-29753(2001)
- Mehle A., Goncalves J., Santa-Marta M.Genes Dev. 18:2861-2866(2004)
- Kamura T., Maenaka K., Kotoshiba S.Genes Dev. 18:3055-3065(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rb
Immunogen:
This CUL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 747-774 amino acids from the C-terminal region of human CUL5.
NCBI Gene ID #:
8065
NCBI Official Name:
Cullin-5
NCBI Official Symbol:
CUL5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
91 kDa
Protein Accession #:
Q93034
Protein GI Number:
14917099
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
Q93034
User NOte:
Optimal dilutions for each application to be determined by the researcher.