RSPO2 Antibody

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ProSci
Product Code: PSI-61-862
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-862-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from 293 cell line, using RSPO2 Antibody at 1:1000 at each lane.
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RSPO2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of lysate from 293 cell line, using RSPO2 Antibody at 1:1000 at each lane.
RSPO2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
R-spondin-2, Roof plate-specific spondin-2, hRspo2, RSPO2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM 116806) signaling.
Background References:
  • Kim, K.A., et al. Cell Cycle 5(1):23-26(2006)
  • Kazanskaya, O., et al. Dev. Cell 7(4):525-534(2004)
  • Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This RSPO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-218 amino acids from the C-terminal region of human RSPO2.
NCBI Gene ID #:
340419
NCBI Official Name:
R-spondin-2
NCBI Official Symbol:
RSPO2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
Q6UXX9
Protein GI Number:
147744588
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q6UXX9
User NOte:
Optimal dilutions for each application to be determined by the researcher.