DENND1B Antibody

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ProSci
Product Code: PSI-55-155
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-155-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:500 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 4
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
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Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western Blot at 1:500 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
DENN domain-containing protein 1B, Connecdenn 2, Protein FAM31B, DENND1B, C1orf218, FAM31B
Application Note:
For WB starting dilution is: 1:500

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).
Background References:
  • Marat, A.L., et al. J. Biol. Chem. 285(14):10627-10637(2010)
  • Sleiman, P.M., et al. N. Engl. J. Med. 362(1):36-44(2010)
  • Venter, J.C., et al. Science 291(5507):1304-1351(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This DENND1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the N-terminal region of human DENND1B.
NCBI Gene ID #:
163486
NCBI Official Name:
DENN domain-containing protein 1B
NCBI Official Symbol:
DENND1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
Q6P3S1
Protein GI Number:
74749089
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q6P3S1
User NOte:
Optimal dilutions for each application to be determined by the researcher.