RCE1 Antibody

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ProSci
Product Code: PSI-61-387
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-387-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of RCE1 using rabbit polyclonal RCE1 Antibody (W57) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCE1 gene.
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RCE1 Antibody (N-term W57) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RCE1 Antibody (N-term W57) for immunohistochemistry.
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Western blot analysis of RCE1 using rabbit polyclonal RCE1 Antibody (W57) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCE1 gene.
RCE1 Antibody (N-term W57) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RCE1 Antibody (N-term W57) for immunohistochemistry.

Documents

Further Information

Additional Names:
CAAX prenyl protease 2, 3422-, Farnesylated proteins-converting enzyme 2, FACE-2, Prenyl protein-specific endoprotease 2, RCE1 homolog, hRCE1, RCE1, FACE2, RCE1A, RCE1B
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
RCE1 is an integral membrane protein which is classified as a member of the metalloproteinase family. This enzyme is thought to function in the maintenance and processing of CAAX-type prenylated proteins.
Background References:
  • Maske, C.P., et al., J. Cell Biol. 162(7):1223-1232 (2003).
  • Hollander, I.J., et al., Biochim. Biophys. Acta 1649(1):24-29 (2003).
  • Freije, J.M., et al., Genomics 58(3):270-280 (1999).
  • Otto, J.C., et al., J. Biol. Chem. 274(13):8379-8382 (1999).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This RCE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-71 amino acids from the N-terminal region of human RCE1.
NCBI Gene ID #:
9986
NCBI Official Name:
CAAX prenyl protease 2
NCBI Official Symbol:
RCE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
Q9Y256
Protein GI Number:
13431529
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9Y256
User NOte:
Optimal dilutions for each application to be determined by the researcher.