TRIM50 Antibody

ProSci
Product Code: PSI-61-996
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-996-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Jurkat cell line lysates (15ug/lane).
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TRIM50 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in Jurkat cell line lysates (15ug/lane).
TRIM50 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
E3 ubiquitin-protein ligase TRIM50, 632-, Tripartite motif-containing protein 50, TRIM50, TRIM50A
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
TRIM50 belongs to the TRIM/RBCC family. It contains 1 B box-type zinc finger, 1 B30.2/SPRY domain and 1 RING-type zinc finger. TRIM50 is an E3 ubiquitin-protein ligase.
Background References:
  • Micale, L., et al. Eur. J. Hum. Genet. 16(9):1038-1049(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TRIM50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354-383 amino acids from the C-terminal region of human TRIM50.
NCBI Gene ID #:
135892
NCBI Official Name:
E3 ubiquitin-protein ligase TRIM50
NCBI Official Symbol:
TRIM50
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
55 kDa
Protein Accession #:
Q86XT4
Protein GI Number:
56404881
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q86XT4
User NOte:
Optimal dilutions for each application to be determined by the researcher.