ITIH2 Antibody

ProSci
Product Code: PSI-62-419
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-419-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of ITIH2 antibody in HepG2 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ITIH2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of ITIH2 antibody in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ITIH2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Inter-alpha-trypsin inhibitor heavy chain H2, ITI heavy chain H2, ITI-HC2, Inter-alpha-inhibitor heavy chain 2, Inter-alpha-trypsin inhibitor complex component II, Serum-derived hyaluronan-associated protein, SHAP, ITIH2, IGHEP2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
ITIH2 may act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
Background References:
  • Obayashi,Y., Oncol. Rep. 19 (5), 1245-1251 (2008)
  • Kishida,T., Connect. Tissue Res. 49 (2), 105-108 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig
Immunogen:
This ITIH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 858-886 amino acids from the C-terminal region of human ITIH2.
NCBI Gene ID #:
3698
NCBI Official Name:
Inter-alpha-trypsin inhibitor heavy chain H2
NCBI Official Symbol:
ITIH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
106 kDa
Protein Accession #:
P19823
Protein GI Number:
229462889
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
P19823
User NOte:
Optimal dilutions for each application to be determined by the researcher.