FBXL17 Isoform 2 Antibody

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ProSci
Product Code: PSI-61-047
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-047-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H. kidney section using FBXL17 Isoform 2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of MCF-7 cells using FBXL17 Isoform 2 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded M. kidney section using FBXL17 Isoform 2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
5 / 5
Western blot analysis of lysate from MCF-7 cell line, using FBXL17 Isoform 2 Antibody at 1:1000.
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Immunohistochemical analysis of paraffin-embedded H. kidney section using FBXL17 Isoform 2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of MCF-7 cells using FBXL17 Isoform 2 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded M. kidney section using FBXL17 Isoform 2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysate from MCF-7 cell line, using FBXL17 Isoform 2 Antibody at 1:1000.

Documents

Further Information

Additional Names:
F-box/LRR-repeat protein 17, F-box and leucine-rich repeat protein 17, F-box only protein 13, FBXL17, FBL17, FBX13, FBXO13
Application Note:
For IHC-P starting dilution is: 1:25

For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex (By similarity).
Background References:
  • Ota T.,et al.Nat. Genet. 36:40-45(2004).
  • Bechtel S.,et al.BMC Genomics 8:399-399(2007).
  • Schmutz J.,et al.Nature 431:268-274(2004).
  • Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FBXL17 Isoform 2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 262-297 amino acids from the C-terminal region of human FBXL17 Isoform 2.
NCBI Gene ID #:
64839
NCBI Official Name:
F-box/LRR-repeat protein 17
NCBI Official Symbol:
FBXL17
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
76 kDa
Protein Accession #:
Q9UF56
Protein GI Number:
229462981
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9UF56
User NOte:
Optimal dilutions for each application to be determined by the researcher.