RNF11 Antibody

ProSci
Product Code: PSI-56-726
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-726-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis of RNF11 using rabbit polyclonal RNF11 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RNF11 gene.
2 / 3
RNF11 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3

Western blot analysis of RNF11 using rabbit polyclonal RNF11 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RNF11 gene.
RNF11 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
RING finger protein 11, RNF11
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
The protein encoded by this gene contains a RING-H2 finger motif, which is known to be important for protein-protein interactions. The expression of this gene has been shown to be induced by mutant RET proteins (MEN2A/MEN2B). The germline mutations in RET gene are known to be responsible for the development of multiple endocrine neoplasia (MEN). [provided by RefSeq].
Background References:
  • Santonico, E., et al. Oncogene 29(41):5604-5618(2010)
  • Markson, G., et al. Genome Res. 19(10):1905-1911(2009)
  • Shembade, N., et al. EMBO J. 28(5):513-522(2009)
  • van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This RNF11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-90 amino acids from the Central region of human RNF11.
NCBI Gene ID #:
26994
NCBI Official Name:
RING finger protein 11
NCBI Official Symbol:
RNF11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
17 kDa
Protein Accession #:
Q9Y3C5
Protein GI Number:
21362884
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9Y3C5
User NOte:
Optimal dilutions for each application to be determined by the researcher.