SACA1 Antibody
Code | Size | Price |
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PSI-55-410-400ul | 400ul | £626.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Further Information
Additional Names:
Sperm acrosome membrane-associated protein 1, Sperm acrosomal membrane-associated protein 32, SPACA1, SAMP32
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Background:
The correlation of anti-sperm antibodies with cases of
unexplained infertility implicates a role for these antibodies in
blocking fertilization. Improved diagnosis and treatment of
immunologic infertility, as well as identification of proteins for
targeted contraception, are dependent on the identification and
characterization of relevant sperm antigens. The protein expressed
by this gene is recognized by anti-sperm antibodies from infertile
males. Furthermore, antibodies generated against the recombinant
protein block in vitro fertilization. This protein localizes to the
acrosomal membrane of spermatids and mature spermatozoa where it is
thought to play a role in acrosomal morphogenesis and in sperm-egg
binding and fusion, respectively.
Background References:
- Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010) :
- Levy, D., et al. BMC Med. Genet.SUPPL 1, S3 (2007) :
- Vasan, R.S., et al. BMC Med. Genet.SUPPL 1, S2 (2007) :
- Mungall, A.J., et al. Nature 425(6960):805-811(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SACA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-74 amino acids from the N-terminal region of human SACA1.
NCBI Gene ID #:
81833
NCBI Official Name:
Sperm acrosome membrane-associated protein 1
NCBI Official Symbol:
SPACA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
32 kDa
Protein Accession #:
Q9HBV2
Protein GI Number:
74752784
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9HBV2
User NOte:
Optimal dilutions for each application to be determined by the researcher.