AGBL5 Antibody

ProSci
Product Code: PSI-56-039
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-039-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in K562 cell line lysates (35ug/lane).
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AGBL5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in K562 cell line lysates (35ug/lane).
AGBL5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Cytosolic carboxypeptidase-like protein 5, 3417-, ATP/GTP-binding protein-like 5, AGBL5, CCP5
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50
Background:
AGBL5 has a function in the processing of cytosolic proteins such as alpha tubulin, which is known to be modified by the removal of a C terminal tyrosine. It is expressed in the brain. There are three named isoforms.
Background References:
  • Bailey, S.D., et al. Diabetes Care (2010) In press :
  • Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
  • Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
  • Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This AGBL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-127 amino acids from the N-terminal region of human AGBL5.
NCBI Gene ID #:
60509
NCBI Official Name:
Cytosolic carboxypeptidase-like protein 5
NCBI Official Symbol:
AGBL5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
98 kDa
Protein Accession #:
Q8NDL9
Protein GI Number:
74715354
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q8NDL9
User NOte:
Optimal dilutions for each application to be determined by the researcher.