PCM-1 Antibody

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ProSci
Product Code: PSI-62-944
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-944-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Fluorescent image of A549 cell stained with PCM-1 Antibody . A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).PCM-1 immunoreactivity is localized to Centrosome significantly.
2 / 4
Western blot analysis of PCM-1 antibody in Ramos cell line lysates (35ug/lane)
3 / 4
4 / 4
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Fluorescent image of A549 cell stained with PCM-1 Antibody . A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).PCM-1 immunoreactivity is localized to Centrosome significantly.
Western blot analysis of PCM-1 antibody in Ramos cell line lysates (35ug/lane)
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Pericentriolar material 1 protein, PCM-1, hPCM-1, PCM1
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
PCM-1 is required for centrosome assembly and function. This protein is essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. The protein is required to anchor microtubules to the centrosome.
Background References:
  • Balczon R., Bao L.J. Cell Biol. 124:783-793(1994)
  • Corvi R., Berger N.Oncogene 19:4236-4242(2000)
  • Dammermann A., Merdes A.J. Cell Biol. 159:255-266(2002)
  • Reiter A., Walz C.Cancer Res. 65:2662-2667(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PCM-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 632-661 amino acids from the Central region of human PCM-1.
NCBI Gene ID #:
5108
NCBI Official Name:
Pericentriolar material 1 protein
NCBI Official Symbol:
PCM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
229 kDa
Protein Accession #:
Q15154
Protein GI Number:
296439495
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
Q15154
User NOte:
Optimal dilutions for each application to be determined by the researcher.