TRIM65 Antibody

ProSci
Product Code: PSI-56-056
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-056-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
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TRIM65 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
TRIM65 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Tripartite motif-containing protein 65, TRIM65
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
TRIM65 belongs to the TRIM/RBCC family. It contains one B box-type zinc finger, one B30.2/SPRY domain and one RING-type zinc finger.
Background References:
  • Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TRIM65 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-355 amino acids from the Central region of human TRIM65.
NCBI Gene ID #:
201292
NCBI Official Name:
Tripartite motif-containing protein 65
NCBI Official Symbol:
TRIM65
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
Q6PJ69
Protein GI Number:
296453004
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q6PJ69
User NOte:
Optimal dilutions for each application to be determined by the researcher.