ZP3 Antibody
| Code | Size | Price |
|---|
| PSI-59-051-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Zona pellucida sperm-binding protein 3, Sperm receptor, ZP3A/ZP3B, Zona pellucida glycoprotein 3, Zp-3, Zona pellucida protein C, Processed zona pellucida sperm-binding protein 3, ZP3, ZP3A, ZP3B, ZPC
Application Note:
For WB starting dilution is: 1:1000
Background:
The zona pellucida is an extracellular matrix that
surrounds the oocyte and early embryo. It is composed primarily of
three or four glycoproteins with various functions during
fertilization and preimplantation development. The protein encoded
by this gene is a structural component of the zona pellucida and
functions in primary binding and induction of the sperm acrosome
reaction. The nascent protein contains a N-terminal signal peptide
sequence, a conserved ZP domain, a C-terminal consensus furin
cleavage site, and a transmembrane domain. It is hypothesized that
furin cleavage results in release of the mature protein from the
plasma membrane for subsequent incorporation into the zona
pellucida matrix. However, the requirement for furin cleavage in
this process remains controversial based on mouse studies. A
variation in the last exon of this gene has previously served as
the basis for an additional ZP3 locus; however, sequence and
literature review reveals that there is only one full-length ZP3
locus in the human genome. Another locus encoding a bipartite
transcript designated POMZP3 contains a duplication of the last
four exons of ZP3, including the above described variation, and
maps closely to this gene.
Background References:
- Davila, S., et al. Genes Immun. 11(3):232-238(2010)
- Bansal, P., et al. Biol. Reprod. 81(1):7-15(2009)
- Choudhury, S., et al. J. Reprod. Immunol. 79(2):137-147(2009)
- Chiu, P.C., et al. Biol. Reprod. 79(5):869-877(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ZP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-380 amino acids from the C-terminal region of human ZP3.
NCBI Gene ID #:
7784
NCBI Official Name:
Zona pellucida sperm-binding protein 3
NCBI Official Symbol:
ZP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
P21754
Protein GI Number:
251757420
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Cell Cycle
Swissprot #:
P21754
User NOte:
Optimal dilutions for each application to be determined by the researcher.