FBXO15 Antibody

ProSci
Product Code: PSI-56-413
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-413-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
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FBXO15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
FBXO15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
F-box only protein 15, FBXO15, FBX15
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Members of the F-box protein family, such as FBXO15, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM].
Background References:
  • Lamesch, P., et al. Genomics 89(3):307-315(2007)
  • Jin, J., et al. Genes Dev. 18(21):2573-2580(2004)
  • Tokuzawa, Y., et al. Mol. Cell. Biol. 23(8):2699-2708(2003)
  • Winston, J.T., et al. Curr. Biol. 9(20):1180-1182(1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This FBXO15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-158 amino acids from the N-terminal region of human FBXO15.
NCBI Gene ID #:
201456
NCBI Official Name:
F-box only protein 15
NCBI Official Symbol:
FBXO15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
Q8NCQ5
Protein GI Number:
384872371
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Cell Cycle,Signal Transduction
Swissprot #:
Q8NCQ5
User NOte:
Optimal dilutions for each application to be determined by the researcher.