HHLA2 Antibody

ProSci
Product Code: PSI-55-312
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-312-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: human pancreas lysate Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 4
HHLA2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in MDA-MB453,CEM,HL-60,MDA-MB231 cell line lysates (35ug/lane).

Western Blot at 1:2000 dilution Lane 1: human pancreas lysate Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane.
HHLA2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB453,CEM,HL-60,MDA-MB231 cell line lysates (35ug/lane).

Further Information

Additional Names:
HERV-H LTR-associating protein 2, Human endogenous retrovirus-H long terminal repeat-associating protein 2, HHLA2
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background References:
  • Mager, D.L., et al. Genomics 59(3):255-263(1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HHLA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human HHLA2.
NCBI Gene ID #:
11148
NCBI Official Name:
HERV-H LTR-associating protein 2
NCBI Official Symbol:
HHLA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
Q9UM44
Protein GI Number:
74762781
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Immunology
Swissprot #:
Q9UM44
User NOte:
Optimal dilutions for each application to be determined by the researcher.